Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry

Combined fluorescence in situ hybridization and immunocytochemistry double labeling in whole-mount Manduca sexta tissue.

A novel triple-color detection procedure for brightfield microscopy, combining in situ hybridization with immunocytochemistry.

We describe a fast light microscopic procedure for the simultaneous enzyme cytochemical detection of three different DNA target sequences in contrasting colors in both interphase and metaphase cell preparations. Chromosome-specific DNA probes labeled with either biotin, digoxygenin, or fluorescein were hybridized as a mixture and detected clearly and accurately by precipitates of the horseradis...

Double labeling for whole-mount in situ hybridization in mouse.

1.Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S517S. 2.Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK. 3.Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cel...

Methods for double detection of gene expression. Combined in situ hybridization and immunocytochemistry of histochemistry.

The distribution of two different molecules can be analyzed within the same embryo using the procedures described below. The protocol for combined whole mount in situ hybridization and immunocytochemistry allows for simultaneous detection of mRNA and protein. The protocol for combined whole mount in situ hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and tr...

Labeling of in situ hybridization DNA probes